High Performance Liquid Chromatography (HPLC)
Lecturer: Dr. Shulamit Levin
Ministry of Agriculture – Plant protection
Beit Dagan

Beit Dagan

Topic No. Title:
1. Fundamentals of liquid chromatography
2. Development of methods in HPLC – various modes of liquid chromatography
3. Reversed phase chromatography- chemistry and geometry of the columns
4. Sample Preparation: Solid Phase Extraction (SPE)
5. Detection in liquid chromatography: General guidelines UV, Fluorescence, Mass-spectrometry, Refractive Index and Electrochemical detectors
6. Photo-diode-array (PDA) detectors: Principle of operation, Peak purity, Spectral matching
7. Liquid chromatography – mass spectrometry (LC-MS) for the positive identification of unknown compounds
8. Gradient conditions – Principles and guidelines
1 . Peak integration in HPLC – Integration events and calibration methods
10. 2 . Validation of chromatographic methods – System suitability, precision, accuracy, limit of detection, limit of quantification etc.
11. 3. Analysis of impurities and degradation products – “working in the ppm world”
12. Preparative chromatography – considerations in scaling up a separation
13. System peaks in liquid chromatography – Their origin and formation, how to live with/without them
14. Chiral chromatography for the analysis of enantiomers
15. Analysis of ions – Ion Chromatography (IC) and Capillary Ion Analysis (CIA)
16. Analysis of polymers – Gel Permeation Chromatography (GPC) for the determination of molecular weight distribution