Modes of liquid chromatography סוגי כרומטוגרפיה נוזלית: הרצאה
Home»Modes of liquid chromatography סוגי כרומטוגרפיה נוזלית: הרצאה
High Performance Liquid Chromatography (HPLC) is an analytical technique for the separation and determination of organic and inorganic solutes in any samples especially biological, pharmaceutical, food, environmental, industrial, etc. In a liquid chromatographic process a liquid permeates through a porous solid stationary phase and elutes the solutes into a flow-through detector.
The stationary phase is usually in the
form of small-diameter (5-10 mm) uniform particles, packed into a cylindrical column.
The typical column is constructed from a rigid material (such as stainless steel or plastic) and is generally 5-30 cm long and the internal diameter is in the range of1-9 mm.
A high pressure pump is required to force the mobile phase through the column at typical flow rates of 0.1-2 ml/min.
The sample to be separated is introduced into the mobile phase by injection device, manual or automatic, prior to the column.
The detector usually contains low volume cell through which the mobile phase passes carrying the sample components.