HPLC Topics | shula-lc

Contents

Introduction and Overview הקדמה וסקירה כללית
Search and/or Development of HPLC Method חיפוש/פיתוח השיטה לעבודה
Validation of HPLC Method for Quantitative Work ולידציה של השיטה לעבודה כמותית
Quantitative Work
Detectors & Detection סוגי גלאים
Operational Instructions and Troubleshooting הוראות תיפעול ואיתור תקלות

Column: LC Troubleshooting LC-GC Europe – By: John W. Dolan
Preparing the Laboratory for HPLC Work הכנת המעבדה לעבודה

Scale up to Preparative Chromatography כרומטוגרפיה פרפרטיבית
SFC/UPC2
LITERATURE ספרות מקצועית

Introduction and Overview הקדמה וסקירה כללית

Search and/or Development of HPLC Method חיפוש/פיתוח השיטה לעבודה

Where to begin if you need to perform HPLC analysis?
First search in the literature for existing methods, do not re-invent the wheel. Go to Google and search for your compound plus the two pharases “mobile phase” and “liquid chromatography”:

Understanding and selecting the right HPLC column בחירת הקולונה המתאימה:

Now, you got an application note or a method from the literature. Chances are (90%) that you got a reversed phase application for your compound. What is next? There is a huge selection of reversed phase columns, do I need to buy exactly the same column as the literature shows? Not necessarily, there is a term called: equivalent column.

Optimize and validate the HPLC method אופטימיזציה וולידציה של השיטה:

After you set up your HPLC method, you need to test it on known samples, and you need to validate your method to be sure that it will work properly and accurately from now on. The next links describe the process and the reason for optimization and validation of HPLC methods.

Validation of HPLC Method for Quantitative Work ולידציה של השיטה לעבודה כמותית

Once you have the method, make sure to assure that it is appropriate for your specific needs, this is done by validation.

Instrumentation Qualification תיקוף מכשירים

Topics related to the hardware of HPLC systems

Quantitative Work

Detectors & Detection סוגי גלאים

General introduction: books, presentations and overviews

Light Spectroscopy Detectors

Aerosol Detectors

Electrochemical & Conductivity Detectors

Mass Spectrometry Detection

* (User guide of Waters’ detector: Register to website first) to get the following information on each detector:

Theory of operation
Installation procedures
User procedures
Maintenance and troubleshooting procedur

Operational Instructions and Troubleshooting הוראות תיפעול ואיתור תקלות

Now that you have a validated method, you start working daily, now you need to know how to operate the instrument, chemistry and software smoothly and trouble-free. For instructions see Operational Instructions

The following are topics related to the proper operation of the HPLC system, chemistry, fluidics, software, plumbing, etc.